C2868
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 | Immunoblotting Tissue extracts and whole cell lysates were separated on SDS-PAGE and blotted with Anti-CRMP1 (Cat. No. C2868). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:1,000 Lanes 1,6: Mouse brain (S1 fraction) 2: Rat brain (S1 fraction) 3: HeLa cells 4: HEK-293T cells transfected with human CRMP1 5: Untransfected HEK-293T cells 6: Inhibition with CRMP1 immunizing peptide (human 472-490) (10 μg/mL) |
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C2868
Sigma
Anti-CRMP1 antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-Collapsin Response Mediator Protein 1, Anti-Dihydropiraminidase-like 1 (DPYSL1), Anti-Dihydropyriminidase Related Proterin-1 (DRP1, DRP-1) |
Description
| Immunogen | synthetic peptide corresponding to amino acids 472-490 located near the C-terminus of human CRMP1, conjugated to KLH. This sequence is identical in mouse and rat CRMP1. |
| Physical form | Solution in 0.01 M phosÂphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~62 kDa |
| species reactivity | human, mouse, rat |
| concentration | ~1.5 mg/mL |
| application(s) | immunoblotting: 1-2 μg/mL using HeLa cells lysate and mouse brain extract (S1 fraction) |
| Gene Information | human ... CRMP1(1400) |
| rat ... Crmp1(25415) | |
| mouse ... Crmp1(12933) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
Related Products
| Related product | C4118, Anti-CRMP1 (N-terminal) antibody produced in rabbit |
